Studies on anti-glycating effects of three varieties of
finger millet (Eleusine coracona)
G. Balaji
Anna University, Chennai, Tamil Nadu
Guided by
G. Bhanuprakash Reddy
ICMR-National Institute of Nutrition
Abstract
Diabetes and its associated pathologies has been a major speculation among growing
population in this world. Globally, an estimated 422 million adults were living with diabetes
in 2014, compared to 108 million in 1980. The global prevalence of diabetes has nearly
doubled since 1980, rising from 4.7 to 8.5% in the adult population. The prevalence of
diabetes is increasing as it would reach 300 million people worldwide in 2025 (WHO report
2016). Protein glycation due to Maillard reaction which sparks the formation of advanced
glycation end products is one of the major cause for the diabetic associated pathological
complications (Chi-Hao Wu et al., 2011). Inhibition of formation of AGE’s ameliorate
complications of diabetics such as neuropathy, nephropathy, retinopathy, cardio myopathy
etc (Singh et al.). Traditionally various whole cereal diet and also herbal supplements has
been long followed to prevent such diabetic complications. These Nutraceuticals and
functional foods have gained much importance in present world due to its potential
therapeutic values as it has propitious effect in alleviating diabetes. Finger millet one of the
minor cereal variety grown almost all parts of the country shows pacifying response in
allaying diabetic associated complications. This finger millet encompasses wide range
dietary fibres, carbohydrates with low glycaemic index and higher sustaining power, high
phytochemicals such as polyphenols, flavonoids, antioxidants etc. Recent studies of finger
millet on animal model manifests propitious effect of polyphenols in type 2 diabetes
(Shobana et al., 2010). In this study three different varieties of finger millet such as CO9,
CO14, GPU28 were chosen for investigation. Invitro AGE’s were synthesized with Bovine
serum albumin (BSA) and Methyl Glyoxal. In vitro analytical methods such as AGE and
tryptophan fluorescence, western blot, protein carbonyls, metal chelating activity, DPPH
assay, AR inhibitory assay (Saraswat et al., 2018) were employed. This study evidenced
salubrious effect of these three-millet’s polyphenolic extracts to be type 1 and type 2